Molecular tracking of insect dispersal to verify arthropod predator movement from an alfalfa field to a peach orchard

Implementation of landscape approaches to conservation biological control programs requires the confirmation putative sources that contribute predator colonization crops. This study aims confirm dispersal from an alfalfa field a neighboring peach orchard with DNA mark-capture procedure based on topical application solution grinded brine shrimp cysts, Artemia spp. (Anostraca: Artemiidae), followed by conventional PCR. To optimize marking procedure, well-known present in orchards as well arable crops, Orius laevigatus (Fieber) (Hemiptera: Anthocoridae), was used model this study. In greenhouse trials, acquisition and retention time markings were determined, either directly spraying them or indirectly via residual contact caged plants after spray. The mark remained detectable O. 6 days, 50% tested predators positive 3 days walking sprayed plants. After that, 25m2 strip crop just cuts, several common species collected using sticky traps placed between both PCR analysis specific primers, 32% analyzed (coccinellids, anthocorids, chrysopids, mirids) showed mark. results usefulness method monitor agents case peach.